Antiphase dynamics in a multimode semiconductor laser with optical injection

A detailed experimental study of antiphase dynamics in a two-mode semiconductor laser with optical injection is presented. The device is a specially designed Fabry-Perot laser that supports two primary modes with a THz frequency spacing. Injection in one of the primary modes of the device leads to a rich variety of single and two-mode dynamical scenarios, which are reproduced with remarkable accuracy by a four dimensional rate equation model. Numerical bifurcation analysis reveals the importance of torus bifurcations in mediating transitions to antiphase dynamics and of saddle-node of limit cycle bifurcations in switching of the dynamics between single and two-mode regimes.Comment: 7 pages, 9 figure

Molecular MRI with hyperpolarized xenon and smart sensors

The main aim of this project was to develop an understanding of both the theoretical and experimental aspects of MRI and NMR spectroscopy, while also gaining expertise in hyperpolarized xenon MRI methods. The first chapter will introduce the basics of NMR required to understand the work presented in subsequent chapters. Chapter 2 explores the area of spectroscopic biomarkers for certain pathologies, including those linked to neuroinflammation and a pilot study was performed examining the effect of induced neuroinflammation on metabolite levels in the mouse brain. Neuroinflammation plays a key role in a wide range of brain disorders including chronic pain, stroke, Parkinson's and Alzheimer's disease. The purpose of this pilot study was to investigate how the concentration levels of certain metabolites believed to be biomarkers of neuroinflammation (in particular myo-inositol) can be monitored non-invasively using MR spectroscopy and thus assist in validating them as biomarkers for neuroinflammation. This gives us specificity, one of the key aims for molecular imaging. Ultimately this may lead to an increased understanding of the disease mechanisms and assist in monitoring disease progression. Chapter 3 contains results from a D2O relaxometry study. Through this work a greater understanding of the fundamentals of NMR relaxation measurements was gained. It also lead to an increased understanding the role of contrast agents for MRI. Contrast agents can improve the relative difference in signal intensity between two adjoining tissues. This is important as sometimes distinction between a region of interest and its surrounding environment is impossible due to tissue properties being too similar or cancellation of signals by space averaging. Chapter 4 contains an overview of current developments in hyperpolarized xenon biosensors. MRI suffers from an inherent lack of sensitivity compared to other imaging modalities, something which can be overcome in hyperpolarization via optical pumping. The concept of a hyperpolarized xenon biosensor along with some relevant background theory will be presented here. Finally, conclusions on the research conducted will be presented along with a description of a future project being carried out which is of great interest due to its relevance to work presented here

Detection of Giardia and helminths in Western Europe at local K9 (canine) sites (DOGWALKS Study)

Background Intestinal parasite contamination from infected dogs can place other dogs and humans at risk. A study was initiated to estimate the prevalence of canine intestinal parasitism by collecting fecal samples in cities across Western Europe. Methods Fresh fecal samples were collected from 2469 dogs visiting 164 parks in 33 cities across 12 countries. Each owner responded to a questionnaire focusing on their dog’s signalment and recent anthelmintic treatment history. The collected samples were examined for hookworms, whipworms, ascarids and Giardia using a coproantigen diagnostic immunoassay and microscopy following centrifugal flotation. Results Nematodes or Giardia were detected in at least one sample from 100% of cities and in 93.3% of parks. Nematodes were detected in 57% of parks. Overall, 22.8% of dogs tested positive for an intestinal parasite, with Giardia being the most commonly identified parasites (17.3% of dogs, 83.5% of parks). For nematode infection, 7.6% of all dogs tested positive, with 9.9% of dogs aged  1 month had passed since the previous dose. Conclusions The prevalence estimates of intestinal parasite infections in dogs reported here highlight the need for owner education concerning guidelines for regular testing and treatment, even in older dogs. Failure to adhere to guidelines can result in ongoing transmission of these infections, including those with zoonotic potential. Combining coproantigen immunoassay with centrifugal flotation for diagnostic testing and regular anthelmintic treatment are important measures for ensuring optimal intestinal parasite control

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymenas germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.</p

Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine ruminal epithelium

peer-reviewedCompensatory growth (CG) is utilised worldwide in beef production systems as a management approach to reduce feed costs. However the underlying biology regulating the expression of CG remains to be fully elucidated. The objective of this study was to examine the effect of dietary restriction and subsequent re-alimentation induced CG on the global gene expression profile of ruminal epithelial papillae. Holstein Friesian bulls (n = 60) were assigned to one of two groups: restricted feed allowance (RES; n = 30) for 125 days (Period 1) followed by ad libitum access to feed for 55 days (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n = 30). At the end of each period, 15 animals from each treatment were slaughtered and rumen papillae harvested. mRNA was isolated from all papillae samples collected. cDNA libraries were then prepared and sequenced. Resultant reads were subsequently analysed bioinformatically and differentially expressed genes (DEGs) are defined as having a Benjamini-Hochberg P value of <0.05. During re-alimentation in Period 2, RES animals displayed CG, growing at 1.8 times the rate of their ADLIB contemporary animals in Period 2 (P < 0.001). At the end of Period 1, 64 DEGs were identified between RES and ADLIB, with only one DEG identified at the end of Period 2. When analysed within RES treatment (RES, Period 2 v Period 1), 411 DEGs were evident. Genes identified as differentially expressed in response to both dietary restriction and subsequent CG included those involved in processes such as cellular interactions and transport, protein folding and gene expression, as well as immune response. This study provides an insight into the molecular mechanisms underlying the expression of CG in rumen papillae of cattle; however the results suggest that the role of the ruminal epithelium in supporting overall animal CG may have declined by day 55 of re-alimentation.SMW received financial assistance from Science Foundation Ireland (SFI) contract no 09/ RFP/GEN2447

Testing Gait with Ankle-Foot Orthoses in Children with Cerebral Palsy by Using Functional Mixed-Effects Analysis of Variance

Dr Morrissey is part funded by the NIHR/HEE Senior Clinical Lecturer scheme. Tis report presents independent research part-funded by the National Institute for Health Research (NIHR) CAT SCL-2013-04-00

Building a conceptual framework for determining individual differences of accessibility to tourist attractions

This paper introduces a conceptual framework for determining individual differences of accessibility to tourist attractions. The framework includes three components: factors affecting accessibility to tourist attractions; the individual characteristics of tourists, such as age, gender; and other explanatory variables that assist in explaining why accessibility to tourist attractions varies. This research indicates that measures of accessibility should include not only commonly used factors such as socio-demographic variables and distance, but also the facilities available at various attractions, management and operational aspects relating to the attraction (what we term "functions"), and the infrastructure used to move between specific attractions (what we term "connectivity of networks"). A case study of the Ningaloo Coast region was conducted to identify individual difference in evaluation of accessibility to tourist attractions. We show that the evaluation of accessibility varies among tourist groups due to their spatial abilities, individual values and needs, and preparatory set

Nanostructural Diversity of Synapses in the Mammalian Spinal Cord

This work for funded by the Biotechnology and Biological Sciences Research Council (BBSRC; BB/M021793/1), RS MacDonald Charitable Trust, Motor Neurone Disease (MND) Association UK (Miles/Apr18/863-791), the Engineering and Physical Sciences Research Council (EPSRC; EP/P030017/1), Welcome Trust (202932/Z/16/Z), European Research Council (ERC; 695568) and the Simons Initiative for the Developing Brain.Functionally distinct synapses exhibit diverse and complex organisation at molecular and nanoscale levels. Synaptic diversity may be dependent on developmental stage, anatomical locus and the neural circuit within which synapses reside. Furthermore, astrocytes, which align with pre and post-synaptic structures to form “tripartite synapses”, can modulate neural circuits and impact on synaptic organisation. In this study, we aimed to determine which factors impact the diversity of excitatory synapses throughout the lumbar spinal cord. We used PSD95-eGFP mice, to visualise excitatory postsynaptic densities (PSDs) using high-resolution and super-resolution microscopy. We reveal a detailed and quantitative map of the features of excitatory synapses in the lumbar spinal cord, detailing synaptic diversity that is dependent on developmental stage, anatomical region and whether associated with VGLUT1 or VGLUT2 terminals. We report that PSDs are nanostructurally distinct between spinal laminae and across age groups. PSDs receiving VGLUT1 inputs also show enhanced nanostructural complexity compared with those receiving VGLUT2 inputs, suggesting pathway-specific diversity. Finally, we show that PSDs exhibit greater nanostructural complexity when part of tripartite synapses, and we provide evidence that astrocytic activation enhances PSD95 expression. Taken together, these results provide novel insights into the regulation and diversification of synapses across functionally distinct spinal regions and advance our general understanding of the ‘rules’ governing synaptic nanostructural organisation.Publisher PDFPeer reviewe